Open AccessEclosion hormone activates phosphatidylinositol hydrolysis in silkworm abdominal ganglia during adult metamorphosis
Author(s) -
SHIBANAKA Yasuhiko,
HAYASHI Hideaki,
TAKAI Michihiro,
FUJITA Norihisa
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb17567.x
Subject(s) - phosphatidylinositol , hydrolysis , phospholipase c , bombyx mori , medicine , inositol , endocrinology , phospholipase , metamorphosis , biology , incubation , biochemistry , juvenile hormone , signal transduction , microbiology and biotechnology , chemistry , enzyme , hormone , larva , receptor , botany , gene
Eclosion hormone (EH), an insect neuropeptide, stimulated phosphatidylinositol (PtdIns) hydrolysis in abdominal ganglia isolated from Bombyx mori in a specific stage of adult development. Incubation of abdominal ganglia from silkworm pharate adults with EH led to an increase in formation of inositol 1,4,5‐trisphosphate but this increase took place transiently, maximum increase being observed 30 s after the addition of EH. PtdIns hydrolysis was stimulated by exogenous EH in a dose‐dependent fashion and was completely abolished by the phospholipase C inhibitors, neomycin and compound 48/80. The EH‐induced PtdIns hydrolysis developed in parallel to the EH‐induced eclosion behaviour during development of the adult. These results suggest that the EH‐stimulated PtdIns Hydrolysis plays an important role in EH‐mediated signal transduction during adult development of B. mori.