Open AccessCharacterization of the chicken α1(VI) collagen promoter
Author(s) -
KOLLER Erich,
TRUEB Beat
Publication year - 1992
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1992.tb17246.x
Subject(s) - promoter , microbiology and biotechnology , gene , housekeeping gene , transcription (linguistics) , biology , response element , caat box , dna , genomic dna , reporter gene , genetics , gene expression , linguistics , philosophy
The promoter of the chicken α1(VI) collagen gene resembles the 5′‐flanking regions of many housekeeping genes. It lacks a canonical TATAA box but contains potential binding sites for transcription factors AP1 and SP1. The promoter region has a relatively high GC content and forms a typical CpG island. In accordance with the absence of a TATAA element, the gene contains multiple transcription‐initiation sites distributed over 80 bp genomic DNA. A 621‐bp fragment derived from the 5′ end of the α1(VI) collagen gene is able to direct transcription of a heterologous reporter gene in transient‐expression assays. Other DNA fragments that are either shorter or longer than the 621‐bp fragment show markedly reduced promoter activity. Thus, the basic promoter element of the α1(VI) collagen gene must reside within this 621‐bp fragment.