Open AccessA rat cerebellar protein containing the cdc10/SWI6 motif
Author(s) -
TAOKA Masato,
YAMAKUNI Tohru,
SONG SiYoung,
YAMAKAWA Yoshio,
SETA Kazuo,
OKUYAMA Tsuneo,
ISOBE Toshiaki
Publication year - 1992
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1992.tb17088.x
Subject(s) - biology , caenorhabditis elegans , peptide sequence , amino acid , cerebellum , biochemistry , drosophila melanogaster , microbiology and biotechnology , neuroscience , gene
Systematic analysis of soluble proteins in developing rat cerebellum by an automated two‐dimensional liquid‐chromatography system detected a number of proteins which increased transiently during the initial stage of postnatal development. One of the proteins, V‐1 was isolated using a liquid‐chromatography system, and its amino acid sequence was determined by analysis of the purified protein. The sequence showed that the V‐1 protein consisted of 117 amino acids with an acetylated N‐terminus, and has 2.5 internal sequence repeats of 33 amino acids. Computer retrieval of the sequence indicated that the repeated sequences have a structural characteristics of the cdc10/SWI6 motif, which is found in a series of proteins, including those involved in cell‐cycle control and cell‐fate determination in yeast, Drosophila melanogaster and Caenorhabditis elegans . The structure of V‐1, coupled with its controlled expression in early postnatal development, implies a potential role for V‐1 in cerebellar morphogenesis.