Open AccessBinding of histones to Xenopus laevis ribosomal genes with different levels of expression
Author(s) -
DIMITROV Stephan I.,
TATEOSSYAN Hilda N.,
STEFANOVSKY Victor Yu.,
RUSSANOVA Valya R.,
KARAGYOZOV Luchesar,
PASHEV Iliya G.
Publication year - 1992
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1992.tb16719.x
Subject(s) - xenopus , histone , gene , biology , gene expression , expression (computer science) , genetics , ribosomal rna , microbiology and biotechnology , computational biology , computer science , programming language
The association of ribosomal RNA genes with histones as a function of their expression has been studied in Xenopus laevis erythrocytes, where the genes are silent, and tadpoles at stage 40, where these genes are actively transcribed. Isolated nuclei were either treated with formaldehyde or irradiated with an ultraviolet laser to cross‐link proteins to DNA. The covalently linked protein‐DNA complexes were purified by centrifugation through CsCl and immunoprecipitated with antibodies against H1, H2A and H4. DNA from the precipitated complexes was analysed for the presence of ribosomal DNA sequences by hybridization to specific probes. The actively transcribed ribosomal genes from X. laevis embryos are associated with H1, H2A and H4 as are the non‐transcribed genes in the erythrocytes.