Open AccessStructural and immunochemical studies of O‐specific polysaccharide of Proteus vulgaris 5/43 belonging to OX19 group (O‐variants)
Author(s) -
VINOGRADOV Eugeny V.,
KACA Wieslaw,
RÓŻALSKI Antoni,
SHASHKOV Alexander S.,
CEDZYNSKI Maciej,
KNIREL Yuriy A.,
KOCHETKOV Nikolay K.
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb21067.x
Subject(s) - proteus vulgaris , polysaccharide , trisaccharide , antigen , chemistry , biochemistry , proteus , hydrogen fluoride , anhydrous , stereochemistry , carbohydrate conformation , microbiology and biotechnology , biology , escherichia coli , organic chemistry , gene , genetics
O‐specific polysaccharide was obtained on mild acid degradation of lipopolysaccharide of Proteus vulgaris 5/43 belonging to OX19 (O‐variants). It was found to contain d ‐glucose, 2‐acetamido‐2‐deoxy‐ d ‐glucose, and 2‐acetamido‐2,6‐dideoxy‐ l ‐glucose (QuiNAc, N ‐acetyl‐ l ‐quinovosamine) in the ratio of about 1:2:1, the last‐named sugar being rather uncommon for bacterial antigens. A computer‐assisted 13 C‐NMR‐based analysis, methylation analysis, and selective cleavage with anhydrous hydrogen fluoride and dilute hydrochloric acid were applied for structural elucidation of the polysaccharide and the following structure was established: → 2)‐β‐ d ‐Glc p ‐(1 → 6)‐α‐ d ‐Glc p NAc‐(1 → 3)‐α‐ l ‐Qui p NAc‐(1 → 3)‐β‐ d ‐Glc p NAc‐(1 →. Serological studies of the O‐antigen and oligosaccharides derived therefrom revealed the importance of the trisaccharide fragment β‐ d ‐Glc p ‐(1 → 6)‐α‐ d ‐Glc p NAc‐(1 → 3)‐α‐ l ‐Qui p NAc in manifesting the antigenic specificity. Serological cross‐reactions were demonstrated between lipopolysaccharides of P. vulgaris 5/43, 8/44, and OX19 strains.