Open AccessRegulation of transcription of the gene coding for peroxisomal 3‐oxoacyl‐CoA thiolase of Saccharomcyes cerevisiae
Author(s) -
EINERHAND Alexandra W. C.,
VOORNBROUWER Tineke M.,
ERDMANN Ralf,
KUNAU WolfH.,
TABAK Henk F.
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb21056.x
Subject(s) - thiolase , biology , upstream activating sequence , peroxisome , saccharomyces cerevisiae , transcription (linguistics) , regulatory sequence , gene , coding region , promoter , biochemistry , microbiology and biotechnology , transcription factor , gene expression , linguistics , philosophy
Transferring Saccharomyces cerevisiae cells from glucose‐ to oleate‐containing growth media results in a significant increase in the number and volume of peroxisomes. To investigate this proliferation process we studied the transcriptional regulation of the gene coding for peroxisomal 3‐oxoacyl‐CoA thiolase (EC 2.3.1.16) in response to the switch in carbon source. Expression was proved to be repressed during growth on glucose, derepressed during growth on glycerol, and induced during growth on oleate as the sole carbon source. By deletion and mutational analysis of sequences upstream of this gene, we have identified a region which is involved in the regulation of transcription. It is contained within a 52‐base‐pair sequence, UAS T52 (upstream activation sequence thiolase 52), located between 203 and 151 nucleotides upstream of the translational initiation codon. This sequence proved to be required for repression, derepression and induction of transcription, and was able to activate transcription from the truncated version of the heterologous iso‐1‐cytochrome‐ c ( CYCI ) promoter in a similar way as in the wild‐type promoter context. Sequence comparison revealed that the UAS T52 contained a sequence motif (‘β‐oxidation box’, that is very similar to sequences located in the 5′‐upstream regions of the genes coding for two other β‐oxidation enzymes of S. cerevisiae : the peroxisomal acyl‐CoA oxidase and the peroxisomal trifunctional β‐oxidation enzyme of S. cerevisiae . Mutational analysis of the ‘β‐oxidation box’ indicates that this sequence motif acts as a UAS in vivo. Sequence comparison also revealed that just upstream of the ‘β‐oxidation box’, between positions – 213 and – 201, a potential binding site occurred for the yeast multifunctional autonomously replicating sequence binding factor ABF1. Gel‐retardation‐competition experiments indicate that ABF1 binds specifically to this sequence.