Open AccessAdrenocorticotropin‐dependent regulation of glutathione transferase subunit 4 in cultured rat adrenal cells
Author(s) -
MANKOWITZ Louise,
RYDSTRÖM Jan,
DEPIERRE Joseph W.
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb21052.x
Subject(s) - aminoglutethimide , hypophysectomy , glutathione , medicine , endocrinology , protein subunit , glutathione s transferase , transferase , biology , cytochrome p450 , in vivo , enzyme , hormone , biochemistry , metabolism , cancer , microbiology and biotechnology , breast cancer , aromatase , gene
After hypophysectomy, the level of glutathione transferase subunit 4 increases in the adrenal, as well as in the liver, as do those of several other forms of glutathione transferase. This increase in subunit 4 can subsequently be down‐regulated by administration of adrenocorticotropin. The present investigation demonstrates that also in primary cultures of female rat adrenal cells an increase in the level of glutathione transferase subunit 4 (as shown by immunoblotting) occurs in the absence of adrenocorticotropin. When adrenocorticotropin or dibutyryladenosine 3′, 5′‐phosphate was administered to these cells, a down‐regulation of this enzyme level was observed, in agreement with the in vivo situation. This down‐regulation was not affected by aminoglutethimide, an inhibitor of the cholesterol‐side‐chain‐cleavage enzyme (cytochrome P ‐450 scc ) which is the rate‐limiting step in the biosynthesis of steroids. Hence adrenal steroid production is not involved in the down‐regulation of glutathione transferase subunit 4 by adrenocorticotropin.