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Notechis 11′2, a non‐toxic phospholipase A 2 from the venom of Notechis scutatus scutatus
Author(s) -
BOUCHIER Christiane,
BOYOT Philippe,
TESSON Frédérique,
TRÉMEAU Odile,
BOUET Françoise,
HODGSON Darren,
BOULAIN JeanClaude,
MÉNEZ André
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb16399.x
Subject(s) - venom , edman degradation , peptide sequence , biochemistry , amino acid , phospholipase a2 , biology , enzyme , gene
Previously, we deduced the amino acid sequence of a novel phospholipase‐A 2 ‐like protein (PLA 2 ) from the nucleotide sequence of a cDNA isolated from a library prepared from the venom gland of the Australian elapid Notechis scutatus scutatus . The corresponding protein has now been identified, purified from the venom and named Notechis 11′2. Its complete amino acid sequence has been determined by automated Edman degradation of both the whole protein and peptides generated by Staphylococcus aureus protease digestion and chemical cleavage at a tryptophan residue. As predicted from its sequence which contains all the residues putatively required for PLA 2 activity, Notechis 11′2 exhibits an esterase activity, preferentially against neutral phospholipids. However, despite its sequence homology with other highly toxic PLA 2 present in the venom of Notechis scutatus scutatus , notechis 11′2 has no lethal activity. This observation further supports the view that the lethal activity of PLA 2 from Notechis scutatus scutatus is not due to the esterasic activity only.

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