Open AccessConstitutive expression of heat‐shock protein 70 in mammalian cells confers thermoresistance
Author(s) -
ANGELIDIS Charalampos E.,
LAZARIDIS Ioannis,
PAGOULATOS Gerassimos N.
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb16088.x
Subject(s) - transfection , hsp70 , heat shock protein , microbiology and biotechnology , gene , biology , chemistry , genetics
A 70‐kDa heat‐shock‐protein (hsp70) expression vector which contains the human hsp70 gene linked to the human β‐actin promoter, was constructed and used to transfect CV1 monkey cells. Stably transfected CV1 clones were isolated which constitutively synthesized increased amounts of hsp70 at normal temperature. It is shown that these clones are resistant to elevated temperature. This finding indicates that hsp70 is involved in the protection of the cells against a lethal heat treatment and maybe responsible for the phenomenon of thermotolerance.