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α‐Factor‐leader‐directed secretion of recombinant human‐insulin‐like growth factor I from Saccharomyces cerevisiae
Author(s) -
STEUBE Klaus,
CHAUDHURI Bhabatosh,
MÄRKI Walter,
MERRYWEATHER James P.,
HEIM Jutta
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb16063.x
Subject(s) - saccharomyces cerevisiae , recombinant dna , biochemistry , molecular mass , yeast , peptide , chemistry , secretion , endoglycosidase h , golgi apparatus , biology , gene , enzyme , cell
A synthetic gene coding for human‐insulin‐like growth factor I (IGFI) was fused to the leader sequence of yeast prepro‐α‐factor and expressed in Saccharomyces cerevisiae under the control of a glyceraldehyde‐3‐phosphate dehydrogenase promoter fragment. Recombinant IGFI was found inside yeast cells and secreted into the medium. The secreted IGFI migrated on SDS gels with the same electrophoretic mobility as authentic IGFI, i.e. at about 7.5 kDa. HPLC analysis of secreted IGFI revealed the presence of the correctly folded, genuine molecule as well as an isomeric byproduct of equal molecular mass but with two of the three disulfide bonds interchanged. Inside exponentially growing cells the 7.5‐kDa IGFI was also found, along with up to four additional IGFI‐related polypeptides of higher molecular mass. By endoglycosidase F treatment the three polypeptides between 19–26 kDa were converted to a single peptide of 17 kDa. Since this peptide also reacted with an anti‐α‐factor antibody, it represents most likely the unglycosylated α‐factor–IGFI fusion precursor. Pulse‐chase experiments established the precursor nature of the intracellular higher‐molecular‐mass IGFI species. Conversion of the primary translation product to the differently glycosylated IGFI precursor proteins and into the mature form occurred very rapidly, within 2 min. Rapid maturation was, however, not followed by an equally rapid secretion of the mature form into the medium: only after 30–40 min did IGFI appear outside the cells. We therefore postulate the presence of an as yet undefined Golgi or post‐Golgi bottleneck representing a major obstacle in secretion of recombinant IGFI from S. cerevisiae cells.

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