Open AccessCharacterization of a novel type of chain‐terminator Galβ1–6Galβ1–4)GlcNAc in an oligosaccharide related to N ‐glycosylated protein glycans isolated from GM 1 the urine of patients with gangliosidosis
Author(s) -
MICHALSKI Jean Claude,
LEMOINE Jérôme,
WIERUSZESKI Jean Michel,
FOURNET Bernard,
MONTREUIL Jean,
STRECKER Gérard
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb16045.x
Subject(s) - oligosaccharide , glycan , chemistry , beta (programming language) , mass spectrometry , carbohydrate conformation , chromatography , biochemistry , disaccharide , nuclear magnetic resonance spectroscopy , stereochemistry , glycoprotein , computer science , programming language
Two new oligosaccharides were isolated from the urine of a patient with GM 1 gangliosidosis. Final purification of the oligosaccharides was accomplished by capillary supercritical fluid chromatography. Structural analysis was by chemical analysis, chemical‐ionization mass spectrometry and 400‐MHz 1 H‐NMR spectroscopy, leading to two primary structures. The first is derived from a classical triantennary N‐acetyllactosamine‐type glycan:The second is unusual with a terminal disaccharide Galβ1‐6Gal, which had not yet been described for glycans of the N‐acetyllactosamine type: