Open AccessHuman complement factor H
Author(s) -
SCHWAEBLE Wilhelm,
SCHWAIGER Helga,
BROOIMANS Ricardo A.,
BARBIERI Antonia,
MÖST Johannes,
HIRSCHKAUFFMANN Monica,
TIEFENTHALER Martin,
LAPPIN David F.,
DAHA Mohamed R.,
WHALEY Keith,
DIERICH Manfred P.
Publication year - 1991
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1991.tb16028.x
Subject(s) - factor (programming language) , complement (music) , factor h , complement system , computer science , biology , immunology , genetics , antibody , programming language , gene , complementation , phenotype
Using cDNA clones H‐19 and H‐46, we have shown previously that three different mRNA species (4.3 kb, 1.8 kb and 1.4 kb) for complement factor H are expressed constitutively in human liver. Here we report data suggesting that the expression of these different factor‐H mRNA species is regulated by tissue‐specific control mechanisms. Total RNA and poly(A)‐enriched RNA from various human tissues (heart, lung, temporal cortex, kidney, spleen, bone marrow and muscle) various cell lines (HepG2, HepG3, HepG4, Hep3B, H‐4, Jurkat, Molt4, H‐9, KHos24Os, A‐431, U937, Mono Mac 6 and Raji) and from primary cultures of peripheral blood monocytes, fibroblasts and human umbilical vein endothelial cells (HUVEC) were investigated for the expression of factor‐H mRNA. In RNA preparations from extrahepatic tissue, factor‐H mRNA was only detected in biopsies from the lung. Using 20 μg total RNA isolated from all 13 cell lines it was not possible to detect any factor‐H mRNA, while mRNA for factor H was expressed in monocytes, HUVEC and fibroblasts. When expressed in extrahepatic tissues, only the 4.3‐kb and the 1.8‐kb mRNA species were detected, while the 1.4‐kb mRNA is expressed abundantly in liver. Interferon‐γ did not induce the expression of factor‐H mRNA in any of the cell lines tested. On the other hand, tumour necrosis factor‐α induced the expression of the 4.3‐kb mRNA species in U937 cells. In HUVEC and fibroblasts the relative quantities of the 4.3‐kb and the 1.8‐kb mRNA species and the regulatory effects of interferon‐γ, interleukin‐1, dexamethasone and retinoic acid on their expression showed significant tissue specificity.