Open AccessTwo‐dimensional NMR studies and secondary structure of cobrotoxin in aqueous solution
Author(s) -
YU Chin,
LEE ChangShin,
CHUANG LiChin,
SHEI YouRhon,
WANG Chi Ying
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb19401.x
Subject(s) - antiparallel (mathematics) , naja , aqueous solution , protein secondary structure , chemistry , beta sheet , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance spectroscopy , stereochemistry , venom , protein structure , organic chemistry , biochemistry , physics , quantum mechanics , magnetic field
The 1 H‐NMR spectra of cobrotoxin, a neurotoxic protein isolated from Formosan cobra Naja naja atra , have been studied by two‐dimensional NMR techniques. Of 62 amino acid residues in cobrotoxin, the complete assignments of 58 residues have been made. The resonances from several of the remaining residues have been identified but not yet specifically assigned. The secondary structure of an antiparallel triple‐ and double‐stranded β‐sheet has also been determined by observing the NOE.