Open AccessStructural and functional characterization of human aromatase P ‐450 gene
Author(s) -
TODA Katsumi,
TERASHIMA Masako,
KAWAMOTO Takeshi,
SUMIMOTO Hidetoshi,
YOKOYAMA Yuichi,
KURIBAYASHI Isao,
MITSUUCHI Yasuhiro,
MAEDA Tomoho,
YAMAMOTO Yasutake,
SAGARA Yusuke,
IKEDA Hisao,
SHIZUTA Yutaka
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb19372.x
Subject(s) - chloramphenicol acetyltransferase , tata box , microbiology and biotechnology , exon , gene , biology , caat box , promoter , acetyltransferase , reporter gene , northern blot , 5' flanking region , transcriptional regulation , gene expression , primer extension , regulatory sequence , genetics , messenger rna , acetylation
The gene encoding aromatase P ‐450 (CYP XIX) has been isolated from two types of human genomic DNA libraries. It spans at least 70 kb and consists of 10 exons. The translational initiation site and the termination site are located in exon 2 and exon 10, respectively. The promoter region of the gene contains a TATA box, a CAAT box and two putative AP‐1 binding sites beginning at –28, –83, –55 and –68 bp, respectively, from the transcriptional initiation site. In addition, a palindromic nucleotide sequence is observed between –209 and –196 and two types of repetitious hexanucleotide (consensus: AATGAA and CCAT AA GG ) are also present within the regions between –485 and –433 and between –358 and –331. Transient expression studies of chloramphenicol acetyltransferase constructs bearing various lengths of 5′‐flanking region of the gene show that the region between –500 and –243 contains negative cis ‐acting element(s), whereas the region between –242 and –183 is required for efficient transcriptional activity. Northern blot analysis demonstrates that the expression of aromatose P ‐450 gene is remarkably stimulated by treatment of cells with 12‐ O ‐tetradecanoyl‐phorbol 13‐acetate. By chloramphenicol acetyltransferase assay the region up to nucleotide position –242 relative to the transcriptional initiation site is shown to participate in the transcriptional responsiveness to this phorbol ester.