Open AccessRecognition of different nucleotide‐binding sites in primary structures using a property‐pattern approach
Author(s) -
Bork Peer,
Grunwald Christian
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb19129.x
Subject(s) - nucleotide , sequence logo , sequence motif , binding site , sequence (biology) , gtp' , consensus sequence , peptide sequence , nad+ kinase , protein primary structure , computational biology , nucleic acid sequence , biology , chemistry , stereochemistry , biochemistry , enzyme , gene
Consensus sequence patterns for β‐α‐β folds binding FAD, NAD and GTP were constructed on the basis of 11 steric and physicochemical properties. These property patterns permit detection and distinction of the respective nucleotide‐binding sites on the basis of amino acid sequence analysis alone. The SWISS‐PROT database (release 9) was screened with the three calculated patterns, and nucleotide‐binding sites identified are presented. They correspond to existing structure data (if known). For the detected sequence segments we are able to predict the β‐α‐β motif as well as the respective binding sites. For some of the proteins so detected a nucleotide‐binding capacity has not previously been reported.