Open AccessStructure and biosynthesis of the xylose‐containing carbohydrate moiety of rice α‐amylase
Author(s) -
HAYASHI Makoto,
TSURU Akiko,
MITSUI Toshiaki,
TAKAHASHI Noriko,
HANZAWA Hiroyuki,
ARATA Yoji,
AKAZAWA Takashi
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb19122.x
Subject(s) - xylose , oligosaccharide , amylase , chemistry , biochemistry , moiety , alpha amylase , biosynthesis , secretion , carbohydrate , stereochemistry , enzyme , fermentation
Suspension‐cultured cells of rice secrete α‐amylase into the culture medium. It has been shown that the mature form of the α‐amylase contains xylose‐bearing N ‐linked oligosaccharide:andWe demonstrate that suspension‐cultured cells of rice secrete α‐amylase containing oligomannose‐type oligosaccharides in the presence of 1‐deoxymannojirimycin or tris(hydroxymethyl)aminomethane. On the other hand, α‐amylase purified from germinated rice seedlings contains several kinds of oligomannose‐type and N ‐acetyllactosamine‐type oligosaccharides. The processing pathway of oligosaccharide moieties in rice cells is discussed on the basis of a comparison of these oligosaccharides structures.