Open AccessStructural analysis of the human inter‐ş‐trypsin inhibitor light‐chain gene
Author(s) -
DIARRAMEHRPOUR Maryam,
BOURGUIG Jeannette,
SESBOÜÈ Richard,
SALIER JeanPhilippe,
LÈVEILLARD Thierry,
MARTIN JeanPierre
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb19102.x
Subject(s) - tata box , exon , intron , gene , genetics , biology , coding region , 5' flanking region , human genome , genomic organization , genome , microbiology and biotechnology , promoter , gene expression
The human inter‐α‐trypsin inhibitor (ITI) light‐chain gene, which codes for the two proteins α 1 ‐microglobulin (protein HC) and ITI‐derived human inhibitor of 30 kDa (HI‐30), was isolated from a human genomic library. This gene, present as a single copy in the human genome, is composed of 10 exons and 9 introns distributed over 20 kbp. A single transcriptional initiation site was identified in the 5′‐flanking region which contained promoter elements, but no typical TATA box. However a sequence equivalent to the TATA box is present on both sense and anti‐sense strands in the 5′‐flanking region of the first exon coding for HI‐3C. The exon‐intron organization suggets that the region coding for protein HC and other members of the lipocalin superfamily evolved from a common ancestral gene that is probabaly differnt from that coding for HI‐30 These data suggest that two distinct ancestral genes could have existed and fused during evolution. Several direct and one inverted repeats are also found within this gene, as well as potential glucorticoid‐receptor binding sites.