Open AccessThe tricarboxylate carrier from rat liver mitochondria
Author(s) -
GLERUM D. Moira,
CLAEYS Dirk,
MERTENS Wim,
AZZI Angelo
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb15668.x
Subject(s) - vesicle , phospholipid , mitochondrion , chemistry , enzyme , biochemistry , kinetics , membrane , physics , quantum mechanics
The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A K m of 40 1tM and a Vmax of 1.56 p.mol × min −1 × mg −1 have been determined for the carrier. Cholesterol levels of between 5–10% of total lipid content are shown to cause a decrease in carrier activity.