Open AccessAnalysis of the regulatory region of the Escherichia coli nupG gene, encoding a nucleoside‐transport protein
Author(s) -
MUNCHPETERSEN Agnete,
JENSEN Nina
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb15608.x
Subject(s) - repressor , escherichia coli , gene , transcription (linguistics) , biology , promoter , regulation of gene expression , microbiology and biotechnology , gene expression , genetics , linguistics , philosophy
The S1‐mapping procedure, when applied to an 800‐bp fragment upstream of the nupG structural gene of Escherichia coli revealed one transcription‐initiation site. The corresponding promoter is negatively regulated by the cytR and the deoR repressors. The expression of the gene is activated by the complex between cAMP and its receptor protein. In strains lacking cAMP the promoter expression is reduced and it is no longer regulated by the cytR repressor, whereas the deoR regulation is retained.