Open AccessDNA sequence of a citrate carrier of Klebsiella pneumoniae
Author(s) -
REST Michel E.,
SCHWARZ Elisabeth,
OESTERHELT Dieter,
KONINGS Wil N.
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb15502.x
Subject(s) - klebsiella pneumoniae , open reading frame , plasmid , dna , escherichia coli , base pair , biology , citric acid , microbiology and biotechnology , gene , peptide sequence , biochemistry
The citrate transport determinant of plasmid pES 1 from Klebsiella pneumoniae [Schwarz E. and D. Oesterhelt (1985) EMBO J. 4 , 1599–1603] has been subcloned in Escherichia coli DH1. The DNA sequence of a 1723‐base fragment that codes for the citrate carrier has been determined and the gene product has been characterized with the T7 promoter system. The DNA fragment contains an open reading frame of 1332 base pairs and codes for a protein of 444 amino acids. The hydropathy profile suggests that the protein is very hydrophobic and contains 12 membrane‐spanning segments centered around a hydrophilic core. The gene for the citrate carrier has 66% similarity with a citrate carrier determinant from a naturally occurring plasmid responsible for secondary transport of citrate across the cytoplasmic membrane of E. coli .