Open AccessDistance between the substrate and regulatory reduced coenzyme binding sites of bovine liver glutamate dehydrogenase by resonance energy transfer
Author(s) -
LARK Richard H.,
COLMAN Roberta F.
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb15414.x
Subject(s) - chemistry , binding site , enzyme , glutamate dehydrogenase , protein subunit , stereochemistry , active site , cofactor , substrate (aquarium) , förster resonance energy transfer , biochemistry , glutamate receptor , biology , fluorescence , receptor , ecology , physics , quantum mechanics , gene
Bovine liver glutamate dehydrogenase is known to bind reduced coenzyme at two sites/subunit, one catalytic and one regulatory; ADP competes for the latter site. The enzyme is here shown to be catalytically active with the thionicotinamide analogue of NADPH ([S]NADPH). For native enzyme, ultrafiltration studies revealed that [S]NADPH reversibly occupies about two sites/enzyme subunit in the absence of other ligands; by the addition of ADP, [S]NADPH binding can be limited to one molecule/subunit. The enzyme is irreversibly inactivated by reaction with 4‐(iodoacetamido)salicylic acid (ISA) at lysine 126 within the 2‐oxoglutarate binding site [Holbrook, J. J., Roberts, P. A. & Wallis, R. B. (1973) Biochem. J. 133 , 165–171]. ISA‐modified enzyme binds 1 molecule [S]NADPH/subunit in the absence of ADP, suggesting that reaction at the substrate site blocks binding at the catalytic, but not at the regulatory site. The fluorescence spectrum of ISA‐modified enzyme overlaps the absorption spectrum of [S]NADPH allowing a distance measurement between these sites by resonance energy transfer. [S]NADPH quenches the emission of ISA‐modified enzyme, yielding 3.2 nm as the average distance between sites. ADP competes for the [S]NADPH site but does not affect the fluorescence of ISA‐modified enzyme, indicating that [S]NADPH quenching is attributable to energy transfer rather than to a conformational change. The 3.2 nm thus represents the distance between the 2‐oxoglutarate and reduced coenzyme regulatory sites of glutamate dehydrogenase.