Open AccessCharacterisation of a monoclonal antibody against native human type I collagen
Author(s) -
WERKMEISTER Jerome A.,
RAMSHAW John A. M.,
ELLENDER Graham
Publication year - 1990
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1990.tb15323.x
Subject(s) - collagenase , monoclonal antibody , epitope , subclass , antibody , pepsin , collagen, type i, alpha 1 , connective tissue , type i collagen , microbiology and biotechnology , chemistry , type ii collagen , biochemistry , biology , immunology , pathology , enzyme , extracellular matrix , medicine , endocrinology , arthritis
A monoclonal antibody against a pepsin‐soluble mammalian type I collagen has been produced. This antibody, subclass IgG 1 , K , was specific for type I collagen and did not cross‐react with a range of other collagen types or connective tissue proteins. The epitope recognized by the antibody was dependent upon an intact triple‐helical structure for the collagen, and was shown by rotary shadowing and by immunoblotting of collagenase‐derived fragments to be near the C‐terminal of the pepsin‐soluble collagen. Although the antibody had a low affinity, with K d = 4×10 −7 M, it could be used for immunohistology of tissue sections and for studies of collagen produced by cells in culture. The antibody, which was raised against human collagen, also recognized type I collagens from certain other species, including calf, pig, sheep, goat and dog.