Open AccessATP synthesis coupled to methane formation from methyl‐CoM and H 2 catalyzed by vesicles of the methanogenic bacterial strain Gö1
Author(s) -
PEINEMANN Susanne,
BLAUT Michael,
GOTTSCHALK Gerhard
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb15192.x
Subject(s) - methanogenesis , valinomycin , protonophore , atp synthase , chemiosmosis , electrochemical gradient , chemistry , ionophore , vesicle , biochemistry , stereochemistry , biophysics , methane , membrane potential , enzyme , biology , organic chemistry , membrane
Methanogenesis from methyl‐CoM and H 2 , as catalyzed by inside‐out vesicle preparations of the methanogenenic bacterium strain Gö1, was associated with ATP synthesis. That this ATP synthesis proceeded via an uncoupler‐sensitive transmembrane proton gradient was concluded from the following results:1 Various inhibitors that affected methane formation (e.g. 2‐bromomethanesulfonate) also prevented ATP synthesis. 2 The protonophore 3,5‐di‐ tert ‐butyl‐4‐hydroxybenzylidenemalononitrile, in combination with the K + ionophore valinomycin, inhibited ATP synthesis completely without affecting methanogenesis. 3 The ATP synthase inhibitor diethylstilbestrol inhibited ATP synthesis. 4 Addition of the detergent sulfobetaine inhibited both methane formation and ATP synthesis; the former but not the latter could be restored by adding titanium(III) citrate as electron donor.In addition it was shown that ATP synthesis could also be driven by transmembrane proton gradients artificially imposed on the vesicles. Furthermore net methanogenesis‐dependent ATP formation was shown by measuring [ 32 P]phosphate incorporation.