Open AccessStructure of Proteus mirabilis 027 O‐specific polysaccharide containing amino acids and phosphoethanolamine
Author(s) -
VINOGRADOV Evgeny V.,
KRAJEWSKAPIETRASIK Danuta,
KACA Wiestaw,
SHASHKOV Alexander S.,
KNIREL Yuriy A.,
KOCHETKOV Nikolay K.
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb15161.x
Subject(s) - proteus mirabilis , chemistry , polysaccharide , glucosamine , anhydrous , mass spectrometry , stereochemistry , biochemistry , organic chemistry , chromatography , escherichia coli , gene
The following structure of the repeating unit of the O‐specific polysaccharide of Proteus mirahilis 027 , containing amides of d ‐glucuronic and n‐galacturonic acids with l ‐lysine and l ‐alanine, respectively, N ‐acetyl‐ d ‐glucosamine and phosphoethanolamine, has been determined:The main methods used for structural analysis of the polysaccharide were selective solvolysis with anhydrous hydrogen fluoride and Smith degradation, as well as NMR spectroscopy and mass spectrometry. The immunodominant role of the lateral N ‐acetyl‐ d ‐glucosamine and phosphoethanolamine in manifesting the serological specificity of P. mirubilis 027 has been established.