Open AccessLigand‐induced conformational changes of citrate synthase studied with the fluorescent probe 8‐anilinonaphthalene 1‐sulfonate
Author(s) -
KOLLMANNKOCH Angelika,
EGGERER Hermann
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb15134.x
Subject(s) - citrate synthase , chemistry , atp synthase , conformational change , ligand (biochemistry) , biophysics , biochemistry , enzyme , biology , receptor
Experiments are presented which show that oxaloacetate and analogs thereof with (R)‐malate substructure, on interaction with citrate synthase linked to synthase 8‐anilinonaphthalene 1‐sulfonate (ANS), induce identical conformational changes of a characteristic magnitude. A conformational change of lower magnitude is also produced on binding of CoASH or ATP to citrate synthase · ANS and is completed on addition of oxaloacetate. The significance of these ligand‐dependent conformational changes is discussed.