Open AccessAnalysis of the α‐amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera
Author(s) -
STRASSER Alexander W. M.,
SELK Regine,
DOHMEN R. Jürgen,
NIERMANN Thomas,
BIELEFELD Martina,
SEEBOTH Peter,
TU Guihong,
HOLLENBERG Cornelis P.
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb15069.x
Subject(s) - biology , kluyveromyces lactis , aspergillus oryzae , gene , saccharomyces cerevisiae , nucleic acid sequence , cosmid , yeast , open reading frame , genetics , microbiology and biotechnology , gene product , genomic library , peptide sequence , biochemistry , gene expression , enzyme
We have cloned and characterized the α‐amylase gene ( AMY1 ) of the yeast Schwanniomyces occidentalis . A cosmid gene library of S. occidentalis DNA was screened in Saccharomyces cerevisiae for α‐amylase secretion. The positive clone contained a DNA fragment harbouring an open reading frame of 1536 nucleotides coding for a 512‐amino‐acid polypeptide with a calculated M r of 56 500. The deduced amino acid sequence reveals significant similarity to the sequence of the Saccharomycopsis fibuligera and Aspergillus oryzae α‐amylases. The AMY1 gene was found to be expressed from its original promoter in S. cerevisiae, Kluyveromyces lactis and Schizosaccharomyces pombe leading to an active secreted gene product and thus enabling the different yeast transformants to grow on starch as a sole carbon source.