Open AccessOne‐ and two‐dimensional NMR studies of yeast phosphoglycerate kinase
Author(s) -
FAIRBROTHER Wayne J.,
BOWEN Derrick,
HALL Len,
WILLIAMS Robert J. P.
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb15058.x
Subject(s) - phosphoglycerate kinase , yeast , sequence (biology) , nuclear overhauser effect , chemistry , proton nmr , domain (mathematical analysis) , substrate (aquarium) , nuclear magnetic resonance spectroscopy , crystallography , biochemistry , stereochemistry , biology , enzyme , mathematics , ecology , mathematical analysis
One‐ and two‐dimensional proton NMR studies have been carried out on yeast phosphoglycerate kinase ( M r ∼ 45000) in order to identify amino‐acid spin systems and obtain sequence‐specific assignments. A number of sequence‐specific assignments have been made using a combination of structural information contained in nuclear Overhauser effect spectra and X‐ray crystallographic data. The results of substrate binding studies (both 3‐phosphoglycerate and Mg · ATP), which indicate mutual reorientation of certain assigned aromatic residues in the inter‐domain region of the protein, are discussed.