Open AccessInhibitory effect of nonviable preparations from human immunodeficiency virus 1 on inositol phospholipid metabolism
Author(s) -
MÜLLER Werner E. G.,
REUTER Petra,
KUCHINO Yoshiyuki,
ROOYEN Jan,
SCHRÖDER Heinz C.
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb14940.x
Subject(s) - inositol , inhibitory postsynaptic potential , phospholipid , metabolism , human immunodeficiency virus (hiv) , chemistry , virus , virology , biology , biochemistry , pharmacology , receptor , endocrinology , membrane
Previously it was established [Pahwa, S., Pahwa, R., Saxinger, C., Gallo, R. C. & Good, R. A. (1985) Proc. Natl Acad. Sci. USA 82 , 8198] that nonviable preparations of human immunodeficiency virus 1 (HIV‐1) abolish the proliferative response of human lymphocytes to phytohemagglutinin A. Now we describe that this effect might be, at least partially, due to an impairment of the function of phospholipase C. It was found that addition of HIV‐1 preparation to lymphocytes diminished the stimulation of phosphatidylinositol phosphorylation caused by phytohemagglutinin A. Moreover, this preparation completely abolished the phytohemagglutinin‐A‐stimulated release of inositol trisphosphate and prevented a translocation of protein kinase C from cytosol to membranes. From this data we conclude that nonviable HIV‐1 preparations inhibit the intracellular signalling pathway, leading to a reduced mitogenic response to phytohemagglutinin A, at the level of protein kinase C.