Open AccessProperties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL 3
Author(s) -
ABELLO Jacques,
DAMIEN Catherine,
NEEF Philippe,
TASTENOY Michèle,
HOOGHE Robert,
ROBBERECHT Patrick,
CHRISTOPHE Jean
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb14922.x
Subject(s) - vasoactive intestinal peptide , receptor , adenylate kinase , cyclase , endocrinology , medicine , biology , agonist , chemistry , neuropeptide , biochemistry
1 Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with β 2 ‐adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL 3 cell line of murine T‐cell lymphoma induced by a radiation leukemia virus. 2 The relative potency of VIP‐related peptides to stimulate adenylate cyclase activity was: helodermin > VIP > peptide histidine isoleucinamide. Five VIP analogs inhibited 125 I‐iodo‐VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP > [D‐Asp 3 ]VIP > [D‐Ser 2 ]VIP > [D‐Ala 4 ]VIP = [D‐His 1 ]VIP = [D‐Phe 2 ]VIP. [D‐Phe 2 ]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin‐ and VIP‐stimulated adenylate cyclase activity with a similar K i (0.07–0.10 μM). These data suggest the existence, in this murine T‐cell lymphoma, of VIP receptors of the ‘helodermin‐preferring’ subtype that are coupled to adenylate cyclase.