Open AccessNew dimeric inhibitor of heterologous α‐amylases encoded by a duplicated gene in the short arm of chromosome 3B of wheat ( Triticum aestivum L.)
Author(s) -
SANCHEZMONGE Rosa,
GOMEZ Luis,
GARCÍAOLMEDO Francisco,
SALCEDO Gabriel
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb14893.x
Subject(s) - gene , chromosome , trypsin inhibitor , gene duplication , genetics , amylase , biology , protease inhibitor (pharmacology) , microbiology and biotechnology , biochemistry , trypsin , enzyme , virus , antiretroviral therapy , viral load
A new wheat dimeric α‐amylase inhibitor, designated WDAI‐3, has been characterized. WDAI‐3 is a homodimeric protein active against α‐amylase from human saliva and from the insect Tenebrio molitor , but inactive against that from pig pancreas or against trypsin. Its N‐terminal amino acid sequence is closer to those of the wheat dimeric inhibitors 0.19 and 0.53 (89–91% identical positions in 44 residues) than to that of the monomeric 0.28 inhibitor (69% identical positions). Iha‐BI‐2 , the gene encoding the new inhibitor, is located in the short arm of chromosome 3B, where it is part of an intrachromosomal gene duplication that also codes for the 0.53 inhibitor.