Open AccessSpectroscopic investigations of Panulirus interruptus hemocyanin in the crystalline state
Author(s) -
VOLBEDA Anne,
FEITERS Martin C.,
VINCENT Michael G.,
BOUWMAN Elisabeth,
DOBSON Barry,
KALK Kor H.,
REEDIJK Jan,
HÓL Wim G. J.
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb14775.x
Subject(s) - monoclinic crystal system , hemocyanin , crystallography , crystal (programming language) , electron paramagnetic resonance , spectroscopy , single crystal , molecule , chemistry , crystal structure , hexagonal crystal system , materials science , biology , nuclear magnetic resonance , physics , organic chemistry , quantum mechanics , antigen , computer science , programming language , genetics
Single‐crystal ultraviolet spectroscopy, X‐ray absorption spectroscopy and EPR measurements have been used to examine the oxidation and oxygenation state of the dinuclear copper site of several types of hemocyanin crystals. The crystals contain Panulirus interruptus hemocyanin which forms hexameric molecules with a molecular mass of approximately 470 kDa. Three types of crystals have been investigated. Type‐I monoclinic crystals, which have been used for the X‐ray structure determination, contain virtually only deoxyhemocyanin. Type‐II monoclinic crystals, which are less well ordered than the type‐I crystals, contain a mixture of deoxy, oxy and met forms. Older crystals contain relatively more methemocyanin. A third, hexagonal, crystal form is also partially oxygenated, and, like the type‐II monoclinic form, subject to gradual conversion to methemocyanin.