Open AccessA phosphodiester bridge between two arabinose residues as a structural element of an extracellular glycöprotein of Volvox carteri
Author(s) -
HOLST Otto,
CHRISTOFFEL Volker,
FRÜND Rüdiger,
MOLL Hermann,
SUMPER Manfred
Publication year - 1989
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1989.tb14730.x
Subject(s) - phosphodiester bond , bridge (graph theory) , extracellular , chemistry , biochemistry , arabinose , microbiology and biotechnology , biology , xylose , anatomy , fermentation , rna , gene
The sulphated glycoprotein SSG 185 is the monomeric precursor of a highly aggregated structural element in the extracellular matrix of the multicellular green alga Volvox carteri . A phosphodiester of arabinose was isolated from a saccharide fragment of SSG 185. The structure of this phosphodiester was investigated by methylation analysis, 13 C‐NMR, photometric methods and enzymatic assays and identified as d ‐Ara f ‐5‐phospho‐5‐ d ‐Ara f . The function of this phosphodiester bridge as a crosslink of different carbohydrate chains in SSG 185 is discussed.