Open AccessFidelity of aminoacylation by rat‐liver tyrosyl‐tRNA synthetase
Author(s) -
TAKAHASHI Ryoya,
GOTO Sataro
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14461.x
Subject(s) - aminoacylation , amino acid , enzyme , tyrosine , phenylalanine , biology , transfer rna , biochemistry , amino acyl trna synthetases , chemistry , microbiology and biotechnology , rna , gene
The possibility of change in the rate of misrecognition of amino acids by rat liver tyrosyl‐tRNA synthetase during aging was investigated. Frequency of misrecognition of phenylalanine vs tyrosine was determined at two temperatures by competitive assay using partially purified enzymes. At 25°C, the error frequencies were 5.17 × 10 −8 and 8.24 × 10 −8 in young and old animals, respectively. These values are much below the reported error frequencies for the prokaryotic enzymes: i.e. ∼ 5 × 10 −6 . Although the fidelity of tyrosyl‐tRNA synthetase from old animals appeared to be slightly lower, the difference was not statistically significant. At 37°C, the error frequencies were increased 1.3–1.5‐fold, but again the difference between young and old animals was not significant. To our knowledge, this is the first report in which fidelity of aminoacyl‐tRNA synthetase of animals of various ages has been compared using natural amino acids.