Open AccessAlterations in the d(CpGpT) structure in solution as a result of [PtCl(diethylenetriamine)] + binding
Author(s) -
GARDEREN Carla J.,
ALTONA Cornelis,
REEDIJK Jan
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14436.x
Subject(s) - diethylenetriamine , deoxyribose , adduct , guanosine , guanine , chemistry , stereochemistry , conformational isomerism , nuclear magnetic resonance spectroscopy , population , molecule , crystallography , nucleic acid , nucleotide , biochemistry , organic chemistry , demography , sociology , gene
The trinucleotide d(CpGpT) reacts with [PtCl(dien)]Cl (dien = diethylenetriamine) to yield as a single adduct Pt(dien)[d(CpGpT)‐ N 7(2)]. The structure of this adduct in solution has been analysed with the aid of NMR spectroscopy and compared with that of the unmodified trinucleotide. A change in the population of the S conformer of the guanosine deoxyribose ring and a syn preference of the guanine residue are the most important changes occurring upon platination. As a result the dC‐dG stack disappears, whereas the dG‐dT stack is hardly affected. The CD spectra of both platinated and free d(CpGpT) confirm the different nature of the two molecules.