Open AccessInteraction of the β‐adrenergic receptor with Gs following delipidation
Author(s) -
BENARIE Nissim,
GILEADI Carina,
SCHRAMM Michael
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14326.x
Subject(s) - gtpase , chemistry , receptor , glycerol , gtp' , adrenergic , adrenergic receptor , biochemistry , oleic acid , enzyme
Preparations of β‐adrenergic receptor and Gs from turkey erythrocytes were delipidated by previously developed procedures. Three synthetic phospholipids, dioleoylglycerophosphoethanolamine, dioleoylglycerophosphocholine and dioleoylglycerophosphoserine plus an unphosphorylated lipid, were all required to restore receptor‐mediated activation of Gs by GTP[γS]. The same lipids were necessary for the reconstitution of the isoproterenol‐enhanced GTPase. The requirement for the unphosphorylated lipid could be fulfilled by 1‐monooleoyl glycerol, α‐tocopherol or oleic acid.