Open AccessStructural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of Shigella flexneri
Author(s) -
WEHLER Thomas,
CARLIN Nils I. A.
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14304.x
Subject(s) - shigella flexneri , serotype , lipopolysaccharide , chemistry , monoclonal antibody , glucosamine , disaccharide , residue (chemistry) , shigella , antigen , acetylation , escherichia coli , microbiology and biotechnology , polysaccharide , stereochemistry , biology , biochemistry , antibody , immunology , gene
The chemical structure of the O‐antigen of a proposed new provisional serotype of Shigella flexneri has been determined. Methylation analysis, GLC‐MS, 1 H‐NMR and 13 C‐NMR showed that the linear O‐antigenic polysaccharide is the same as for all S. flexneri [Kenne, L., Lindberg, B., Petersson, K. & Romanowska, E. (1977) Carbohydr. Res. 56 , 363‐370]. A novel structural feature is that the disaccharide α‐D‐Gl cp ‐(1→2)‐α‐D‐Gl cp is linked to O4 of the N ‐acetyl‐glucosamine residue.Western blotting of the lipopolysaccharide with an E. coli R3 core‐specific monoclonal antibody, suggested the presence of an E. coli R3 core.