Open AccessNucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium
Author(s) -
KILSTRUP Mogens,
LU ChungDar,
ABDELAL Ahmed,
NEUHARD Jan
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14299.x
Subject(s) - operon , biology , derepression , microbiology and biotechnology , cytosine , lac operon , nucleotide , nucleic acid sequence , gene , escherichia coli , biochemistry , gene expression , psychological repression
The carAB operon of Salmonella typhimurium encoding carbamoyl‐phosphate synthetase (CPSase) has been cloned, and the nucleotide sequence of the first gene of the operon, carA , together with 760 base pairs of the 5′‐flanking region was determined. The product of the carA gene is the small subunit of CPSase. It catalyzes the transfer of the amide group from glutamine to an NH 3 ‐site on the heavy subunit. Primer extension and S1 niiciease mapping of in vivo carAB transcripts revealed that transcription is similar to that of Escherichia coli [Piette, J. et al. (1984) Proc. Natl Acad. Sci. USA 81 , 4134‐4138] in its initiation at two promoters, P1 and P2, controlled by pyrimidines and arginine, respectively. The arginine control is mediated through binding to the arginine repressor ( argR ). The involvement of titratable regulatory elements is indicated by the escape from both arginine and pyrimidine control, when the operon is present in multicopies on a plasmid. Measurements of CPSase levels in mutants which allows independent manipulation of the intracellular uraoy and cytosine nucleotide pools show, that both uracil and cytosine nucleotides are required for full repression and that limitation of either nucleotide results in derepression of CPSase synthesis. Deletion analyses indicate that regions upstream of the PI promoter are required for normal expression from this promoter but not from P2.