Open AccessExpression in non‐lymphoid cells of mouse recombinant immunoglobulindirected against the tumour marker human placental alkaline phosphatase
Author(s) -
WAELE Peter,
FEYS Véronique,
VOORDE André,
MOLEMANS Francis,
FIERS Walter
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14280.x
Subject(s) - placental alkaline phosphatase , complementary dna , microbiology and biotechnology , recombinant dna , biology , monoclonal antibody , alkaline phosphatase , antibody , immunoglobulin light chain , cell culture , messenger rna , coding region , gene , enzyme , biochemistry , immunology , genetics
From a mouse hybridoma cell line secreting a monoclonal antibody directed against the tumour marker human placental alkaline phosphatase, mRNA coding for the H and L chains of this antibody was isolated and cloned as cDNA. Sequence analysis of the H and L chain cDNAs confirmed the IgG2b, K ; subtype previously established. Recloning the H and L chain cDNA information into SV40‐based vectors enabled us to obtain expression of functional immunoglobulin upon cotransfection into COS or CHO dhfr cells. This illustrates that non‐lymphoid cells also have the capacity to assemble active immunoglobulins.