Open AccessCloning and expression of cDNA for human vascular anticoagulant, a Ca 2+ ‐dependent phospholipid‐binding protein
Author(s) -
MAURERFOGY Ingrid,
REUTELINGSPERGER Chris P. M.,
PIETERS Jean,
BODO Gerhard,
STRATOWA Christian,
HAUPTMANN Rudolf
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14139.x
Subject(s) - complementary dna , recombinant dna , microbiology and biotechnology , cdna library , cloning (programming) , escherichia coli , biology , expression vector , chemistry , gene , biochemistry , computer science , programming language
Based on sequence information from tryptic peptides an almost full‐size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.