Open AccessExpression of a DNA‐ligase‐stimulatory factor in Bloom's syndrome cell line GM1492
Author(s) -
KENNE Kerstin,
LJUNGQUIST Siv
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14121.x
Subject(s) - dna ligase , microbiology and biotechnology , ubiquitin ligase , dna , biology , cell culture , chemistry , biochemistry , genetics , ubiquitin , gene
An increased DNA ligase activity is observed in extracts of Bloom's syndrome (BS) fibroblast cell line GM1492. The activity is 2–3‐fold higher in this cell line compared to normal human fibroblasts, and 5–20‐fold higher than in three other BS cell lines investigated. The DNA ligase activity in GM1492 cells is promoted by a heat‐resistant, protease‐sensitive factor comigrating with DNA ligases on single‐stranded‐DNA–cellulose. The factor stimulates DNA ligase I as well as DNA ligase II, and is not identical to the activity‐promoting homologous DNA pairing, which is also enhanced in GM1492 cell extracts.