Open AccessImmunoelectron microscopic localisation of ribosomal proteins from Bacillus stearothermophilus that are homologous to Escherichia coli L1, L6, L23 and L29
Author(s) -
HACKL Wolfgang,
STÖFFLERMEILICKE Marina
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14116.x
Subject(s) - immunoelectron microscopy , ribosome , ribosomal rna , escherichia coli , ribosomal protein , eukaryotic ribosome , 50s , biology , homologous chromosome , 30s , biochemistry , chemistry , microbiology and biotechnology , genetics , rna , gene , antibody
The locations of proteins BL1, BL6, BL23 and BL29 from Bacillus stearothermophilus have been determined on the ribosomal surface by immunoelectron microscopy. All four proteins were localized in the same region of the 50s subunit as their homologous counterparts from Escherichia coli , indicating that the ribosomal architecture is the same in both species. This finding is of great importance as it allows structural data obtained on ribosomes from either organism to be incorporated into a unique ribosome model.