Open AccessStructural organization of satellite I chromatin of calf liver
Author(s) -
PAGÈS Michel,
ROIZÈS Gérard
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14110.x
Subject(s) - hpaii , satellite dna , nucleosome , satellite , chromatin , dna , cpg site , biology , centromere , genetics , dna methylation , microbiology and biotechnology , chromosome , gene , physics , astronomy , gene expression
The organization of liver calf satellite DNA I chromatin has been studied. Precisely seven nucleosomes per DNA satellite repeat (1.4 kb) are shown to be present. Several strict positionings of the nucleosomes along the satellite DNA repeat have been described. One of these positionings, the major phase, was shown to be preferentially organized in clustered repeats. Most of the non‐methylated CpG restriction sites were shown to be located in the linker DNAs of the satellite I nucleosomes, whilst, accordingly, methylated Hpa II sites were found in the core satellite I DNA. The significance of these results is discussed.