Open AccessThe disaggregation of calcium‐depleted casein micelles
Author(s) -
GRIFFIN Mary C. A.,
LYSTER Richard L. J.,
PRICE John C.
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb14103.x
Subject(s) - micelle , turbidimetry , chemistry , casein , calcium , chromatography , size exclusion chromatography , phosphate , biochemistry , aqueous solution , organic chemistry , enzyme
The effect of depletion of Ca 2+ on the composition and size distribution of casein micelles in milk has been examined using chemical analysis, size exclusion chromatography, fast protein liquid chromatography, turbidimetry and photon correlation spectroscopy. Partial removal of Ca 2+ by EDTA and subsequent dialysis resulted in disaggregation of some of the casein micelles; as the EDTA concentration increased, the proportions of Ca 2+ and phosphate relative to protein in the micelles remaining intact decreased. However, the composition of the intact micelles, with respect to the different caseins, and the number‐frequency size distribution were essentially unchanged.