Open AccessFusion between vesicles from the pathway of receptor‐mediated endocytosis in a cell‐free system
Author(s) -
WOODMAN Philip G.,
WARREN Graham
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb13972.x
Subject(s) - endocytosis , vesicle , endocytic cycle , transferrin , transferrin receptor , fusion , microbiology and biotechnology , chemistry , lipid bilayer fusion , cytosol , receptor mediated endocytosis , fusion protein , cell , biochemistry , biology , membrane , recombinant dna , philosophy , gene , enzyme , linguistics
Fusion between endocytic vesicles containing 125 I‐transferrin and ones containing anti‐transferrin antibody was monitored by the formation of an immune complex. Fusion required ATP and only occurred between specific populations of vesicles. Cytosol was also required. Trypsinisation of the preparations or pre‐treatment with N ‐ethylmaleimide abolished the fusion reaction.