Open AccessPurification of the LSTRA tyrosine protein kinase (p56 lck )
Author(s) -
TAN Huynh,
FISCHER Siegmund,
FAGARD Remi
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb13856.x
Subject(s) - isoelectric focusing , microbiology and biotechnology , polyacrylamide gel electrophoresis , biology , tyrosine , tyrosine kinase , protein kinase a , lymphoma , kinase , biochemistry , enzyme , immunology , signal transduction
p56 lck , a p60 src related tyrosine protein kinase was recently described in a murine lymphoma cell line (LSTRA), in several human lymphomas and in normal peripheral lymphocytes. We have purifed p56 lck to homogeneity by electrofocusing followed by two‐step SDS/polyacrylamide gel electrophoresis. The pure protein was identical to the p56 characterized in LSTRA cells and used to raise antibodies in rabbits. This simple procedure is applicable for the rapid purification of minor proteins.