Open AccessPreferential positioning of nucleosomes on pBR322 as evaluated via Fourier transform of data from electron microscopy
Author(s) -
CAFFARELLI Elisa,
SANTIS Pasquale,
LEONI Luisa,
PALLESCHI Antonio,
SAVINO Maria
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb13817.x
Subject(s) - nucleosome , electron microscope , pbr322 , fourier transform , histone octamer , dna , chemistry , histone , microscopy , biophysics , crystallography , materials science , physics , optics , biology , biochemistry , plasmid , quantum mechanics
Nucleosome positioning on pBR322 DNA has been evaluated by electron microscopy visualization, after psoralen cross‐linking. The distribution function of nucleosomes on pBR322 DNA has been calculated analyzing the data of the electron microscopy via Fourier transform. This function shows definite maxima, which indicate differential interactions of the histone octamer to different DNA sequences.