Open AccessOne‐ and two‐dimensional 1 H‐NMR investigations of two 19‐base‐pair analogues of the tet operator
Author(s) -
STOLARSKI Ryszard,
BUCK Friedrich,
FERA Brian,
RÜTERJANS Heinrich
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13651.x
Subject(s) - base pair , tn10 , nuclear magnetic resonance spectroscopy , chemistry , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , duplex (building) , crystallography , dna , repressor , base (topology) , operator (biology) , cytidine , spectroscopy , proton nmr , spectral line , transposable element , physics , biochemistry , mathematics , mathematical analysis , genome , transcription factor , gene , quantum mechanics , enzyme , astronomy
Two 19‐base‐pair oligodeoxynucleotides, analogues of one of the operators which specifically bind the repressor protein in the regulatory part of the transposon Tn10 tetracycline‐resistance (tet) determinant, have been studied by 1 H‐NMR spectroscopy. The analogues contain a mismatch in the central base pair of the double helix (T.T or A.A). The imino protons have been assigned to the base pairs by one‐dimensional NOE measurements, and the thermally induced transition from the duplex to the single strand has been followed. The cytidine amino resonances have been assigned by means of two‐dimensional NOE spectroscopy in H 2 O. Two‐dimensional phase‐sensitive NOE and magnitude‐correlated spectra have been recorded in 2 H 2 O; all nonexchangeable protons, with the exception of some of H5′, H5” protons, have been assigned. The NMR data made it possible to carry out a qualitative analysis of the structures of both oligodeoxynucleotides. The general structures close to B‐DNA, show irregularities in the mismatch areas.