Open AccessPrimary structure determination of seven novel N‐linked carbohydrate chains derived from hemocyanin of Lymnaea stagnalis
Author(s) -
KUIK J. Albert,
SIJBESMA Rint P.,
KAMERLING Johannis P.,
VLIEGENTHART Johannes F. G.,
WOOD Edward J.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13626.x
Subject(s) - lymnaea stagnalis , chemistry , galactosamine , carbohydrate , xylose , galactose , monosaccharide , protein primary structure , stereochemistry , biochemistry , hemocyanin , biology , ecology , genetics , gene , snail , peptide sequence , antigen , fermentation
Hemocyanin from the freshwater snail Lymnaea stagnalis is a high‐molecular‐mass copper‐containing glycoprotein which functions as oxygen carrier in the hemolymph. To release the carbohydrate chains, the protein was digested by pronase followed by hydrazinolysis and reduction. The oligosaccharide‐alditols were purified by gel permeation chromatography on Bio‐Gel P‐4, followed by HPLC on a Lichrosorb‐NH 2 column. Using 500‐MHz 1 H‐NMR spectroscopy, in conjunction with sugar, methylation and deamination analysis, the following seven novel primary oligosaccharide structures could be unravelled.