Open AccessMutagenesis of murine granulocyte/macrophage‐colony‐stimulating factor reveals critical residues near the N terminus
Author(s) -
GOUGH Nicholas M.,
GRAIL Dianne,
GEARING David P.,
METCALF Donald
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13619.x
Subject(s) - mutagenesis , mutant , site directed mutagenesis , granulocyte macrophage colony stimulating factor , in vitro , microbiology and biotechnology , biology , granulocyte , granulocyte macrophage colony stimulating factor receptor , colony stimulating factor , granulocyte colony stimulating factor receptor , macrophage , transfection , mutation , chemistry , biochemistry , cell culture , macrophage colony stimulating factor , genetics , gene , haematopoiesis , stem cell
A number of cDNAs encoding mutant forms of the murine haemopoietic growth factor, granulocyte/macrophage‐colony‐stimulating factor (GM‐CSF), have been derived by in vitro mutagenesis and expressed in simian COS cells. Determination of the biological activity of the mutant factors revealed that residues within the regions 11–15, 24–37, 47–49 and 81–89 are required for generating a functional GM‐CSF molecule. In particular, truncation of either of two strongly predicted α helices near the N terminus of the molecule severely depresses the activity of the factor.