Open AccessOscillation of ion fluxes in mammalian erythrocytes
Author(s) -
HOLMUHAMEDOV Ehson L.,
TEPLOVA Vera V.,
SADYKOV Yuri H.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13571.x
Subject(s) - ionophore , chemistry , oscillation (cell signaling) , centrifugation , membrane , extracellular , biophysics , incubation , ion , desorption , chromatography , biochemistry , biology , organic chemistry , adsorption
The dependence of ionophore‐induced oscillations in rat erythrocytes on various concentrations of A23187, FCCP and Ca 2+ was analysed using ion‐selective electrodes. The oscillations were shown to be independent of the extracellular concentration of carbonylcyanide p ‐trifluoromethoxyphenylhydrazone and Ca 2+ . The dependence of oscillations on the concentration A23187 was shown to be a threshold characteristic and represented by a bellshaped curve. In the course of oscillations the redistribution of A23187 between cells and the incubation medium was demonstrated using high‐speed centrifugation. A hypothesis for oscillatory‐state generation in erythrocytes was suggested on the basis of pH‐dependent changes of the Ca 2+ ionophore A23187 content in cells. According to this hypothesis the H + concentration within the external membrane‐adjacent layer serves as a causative factor for induction of cyclic desorption of A23187 molecules from the cell membrane.